Recommended usage: Brush twice a day for 2 minutes each time. Children below 6 years of age should have adult supervision. To maintain good oral hygiene brush twice a day for 2 minutes each time, rinse with mouthwash for 30 seconds, and replace your toothbrush every 3 months. Colgate is India's most trusted oral care brand and certified by Indian Dental Association. This toothpaste is Gluten free.